dc.contributor.author |
Vilanova Canet, Bartolomé
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dc.contributor.author |
Adrover Estelrich, Miguel
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dc.contributor.author |
Muñoz Izquierdo, Francisco
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dc.contributor.author |
Donoso Pardo, Josefa Laurentina
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dc.date.accessioned |
2018-11-22T11:33:30Z |
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dc.identifier.uri |
http://hdl.handle.net/11201/148581 |
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dc.description.abstract |
[eng] In this work, we applied multi-wavelength stopped-flow spectroscopy (MSFS) to study the chemical equilibria between tautomeric or hydrated forms of various vitamin B6 compounds and the Schiff base formed by ε-aminocaproic acid (=6-aminohexanoic acid) with pyridoxal 5′-phosphate at 25° and variable pH. Since some of these compounds are photosensitive, we analyzed the possible occurrence of any secondary photo-induced processes under the conditions of irradiation in the MSFS equipment (continuous irradiation with light from a 75-W Xe lamp spanning the wavelength range of 200<br>700 nm). To determine the tautomeric composition of these compounds, the electronic absorption spectra were analyzed by means of log-normal curves. Continuous irradiation of pyridoxamine and pyridoxal 5′-phosphate over the wavelength range of 200<br>700 nm displaces the chemical equilibrium between the tautomeric or hydrated forms of these compounds. However, the Schiff base of ε-aminocaproic acid with pyridoxal 5′-phosphate is insensitive to the radiation used. The photo-induced processes detected in pyridoxamine and pyridoxal 5′-phosphate should be taken into account in examining vitamers by MSFS. In fact, these additional processes should be considered in studying the mechanism of action of vitamin B6-dependent enzymes by the MSFS technique, whenever some free vitamer may be present in solution. |
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dc.format |
application/pdf |
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dc.relation.isformatof |
https://doi.org/10.1002/CBDV.200490080 |
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dc.relation.ispartof |
Chemistry & Biodiversity, 2004, vol. 1, num. 7, p. 1073-1090 |
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dc.rights |
, 2004 |
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dc.subject.classification |
54 - Química |
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dc.subject.other |
54 - Chemistry. Crystallography. Mineralogy |
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dc.title |
Photoinduced processes in vitamin B6 compounds. |
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dc.type |
info:eu-repo/semantics/article |
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dc.date.updated |
2018-11-22T11:33:30Z |
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dc.date.embargoEndDate |
info:eu-repo/date/embargoEnd/2075-01-01 |
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dc.embargo |
2075-01-01 |
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dc.subject.keywords |
Vitamin b6 |
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dc.subject.keywords |
UV-Vis spectroscopy |
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dc.subject.keywords |
stopped-flow |
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dc.rights.accessRights |
info:eu-repo/semantics/embargoedAccess |
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dc.identifier.doi |
https://doi.org/10.1002/CBDV.200490080 |
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