Characterization of the full-length btuB riboswitch from Klebsiella pneumoniae

Show simple item record Palou Mir, Joana Musiari, Anastasia Sigel, Roland Barceló Oliver, Miquel 2018-10-11T12:51:25Z
dc.description.abstract [eng] Riboswitches are cis-regulatory RNA elements on the mRNA level that control the expression of the downstream coding region. The interaction of the riboswitch with its specific metabolite, which is related to the function of the controlled gene, induces a structural change of the RNA architecture. Consequently, gene regulation is induced by un/masking of the ribosome binding site (RBS). In the genome of Klebsiella pneumoniae a sequence was identified by bioinformatics and proposed to be a B12 riboswitch regulated by coenzyme B12. Here we study this new coenzyme B12- dependent riboswitch system by in-line probing and ITC. The riboswitch sequence includes the whole expression platform as well as RBS. In-line probing experiments were performed to investigate the structural rearrangement of this 243-nt long RNA sequence while Isothermal Titration Calorimetry (ITC) yielded the thermodynamic parameters of the interaction between the riboswitch and its metabolite. The interaction of coenzyme B12 with the butB riboswitch of K. pneumoniae is an exothermic process with a 1:1 binding stoichiometry and binding affinities of log KA = 6.73 ± 0.02 at 15 ºC and log KA = 6.00 ± 0.09 at 30 ºC.
dc.format application/pdf
dc.relation.isformatof Versió postprint del document publicat a:
dc.relation.ispartof Journal of Inorganic Biochemistry, 2016, vol. 160, p. 106-113
dc.subject.classification 54 - Química
dc.subject.other 54 - Chemistry. Crystallography. Mineralogy
dc.title Characterization of the full-length btuB riboswitch from Klebsiella pneumoniae
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/acceptedVersion 2018-10-11T12:51:25Z info:eu-repo/date/embargoEnd/2075-01-01
dc.embargo 2075-01-01
dc.subject.keywords RNA
dc.subject.keywords btuB riboswitch
dc.subject.keywords coenzyme B12
dc.subject.keywords in-line probing
dc.subject.keywords ITC
dc.rights.accessRights info:eu-repo/semantics/embargoedAccess

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