[eng] The eukaryotic cell contains two main internal compartments; the cytoplasm and the nucleus,
each bounded by cell membranes. The cytoplasm is isolated from the extracellular medium
by an outer or plasma membrane and the nucleus is separated from the cytoplasm by a
nuclear membrane. These cell membranes besides physically separating both compartments
additionally present functions of vital importance to the cell such as the regulation of their
response to external signals, in order to adapt to these conditions by activating certain cellular
signaling pathways, allowing the cell to stimulate or inactivate the synthesis of certain
proteins involved in cell proliferation, cell cycle and apoptosis. In cancer, these responses
from the cell to external signals are affected, causing aberrant signaling pathways and
consequently the stimulation of the continuous synthesis of certain proteins, which leads to
uncontrolled growth of the cell. The altered signaling pathways in tumor cells have been
attributed in several studies to changes or imbalances in the lipid composition of the plasma
membrane. It exists a new therapeutic strategy denominated Lipid Membrane Therapy
(TLM) which is dedicated to the design of new molecules that modify altered membrane
lipids in tumor cells, thereby regulating the activity of proteins involved in signal
transduction. 2OHOA modifies the lipid composition of the plasma membrane of the cell
causing changes in the activation and localization of proteins involved in signaling
proliferation and differentiation, showing antiproliferative effects. In this study, from
2OHOA-treated glioblastoma U118 cells at different time intervals (24h and 48h) and
compared to an untreated control, we sought to determine whether changes that have been
described in the plasma membrane of tumor cells can be occurring at the nuclear membrane
level and whether these could have an effect on nucleo-cytoplasmic trafficking of
transcription factors whose levels are affected by treatment with 2OHOA. For this, through
cell fractionation, the two main fractions of the cell are separated; cytoplasmic and nuclear,
and to analyze the situation of certain transcriptional factors responsible for regulating gene
expression in the nucleus from cytoplasmic signals such as NICD, c-Jun, E2F -1, β-catenin
and FOXO1 (Forkhead Box O1), in addition to the separation of the different lipid species by
thin layer chromatography (HP-TLC) and to evaluate possible lipid changes in the nuclear
membrane caused by 2OHOA in glioblastoma cells. The results obtained from the lipid
species show a tendency to be altered after the treatment with 2OHOA in the different cell
fractions, and in a significant way the transcription factors are influenced in the nucleuscytoplasmic
traffic affecting possible cell, signaling pathways related to apoptosis, cell cycle
progression and differentiation, among others. Thus, treatment with 2OHOA in U118
glioblastoma cells can induce antiproliferative effects.