Improving effects of long-term growth hormone treatment on monoaminergic neurotransmission and related behavioral tests in aged rats

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dc.contributor.author Esteban Valdés, Susana Cristina
dc.contributor.author Garau, Celia
dc.contributor.author Aparicio Martínez, Sara
dc.contributor.author Moranta Moranta, David
dc.contributor.author Barceló, Pere
dc.contributor.author Ramis Escudero, Margarita Rosa
dc.contributor.author Tresguerres, Jesús A.F.
dc.contributor.author Rial Planas, Rubén
dc.date.accessioned 2018-09-17T09:26:04Z
dc.identifier.uri http://hdl.handle.net/11201/147441
dc.description.abstract [eng] An age-related decline in cognitive functions and physical performance has been associated with reductions in growth hormone (GH) secretion and brain neurotransmitter function. In vivo experiments were performed to study the long-term effects of exogenously administered GH on the central monoaminergic neurotransmitters serotonin, dopamine, and noradrenaline and behavioral tests in old Wistar rats. The accumulation of 5-hydroxytryptophan (5-HTP) and L-3,4-dihydroxyphenylalanine (DOPA) after decarboxylase inhibition was used as a measure of the rate of tryptophan and tyrosine hydroxylation in vivo. Also, the content of the neurotransmitters serotonin, dopamine, and noradrenaline and some metabolites was measured by high-pressure liquid chromatography (HPLC) in the hippocampus and striatum, brain regions involved in adult memory processing and motor coordination. The age-related decline observed in all the neurochemical parameters in control rats was significantly reversed after repeated subcutaneous administration of GH (2mg/kg per day, 4 weeks). Thus, GH treatment exerted a long-term effect on serotonin, dopamine, and noradrenaline neurotransmission by enhancing neurotransmitter synthesis and metabolism in aged rats. The results obtained after examining working memory tasks in the eight-radial maze and motor ability in the Rotarod treadmill in aged rats were consistent with these neurochemical data; both tests were significantly improved after chronic GH treatment. Overall, these in vivo findings suggest that the positive effects induced by GH on serotonin, dopamine, and noradrenaline neurotransmitters might explain, at least in part, the effects of chronic GH treatment in improving cognitive and motor ability in aged rats, and could aid in preventing or delaying deficits in monoamines associated with learning or motor disabilities.
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dc.relation.isformatof Versió postprint del document publicat a: https://doi.org/10.1089/rej.2010.1053
dc.relation.ispartof Rejuvenation Research, 2010, vol. 13, num. 6, p. 707-716
dc.rights (c) Mary Ann Liebert, Inc. publishers, 2010
dc.subject.classification 576 - Biologia cel·lular i subcel·lular. Citologia
dc.subject.classification 612 - Fisiologia
dc.subject.other 576 - Cellular and subcellular biology. Cytology
dc.subject.other 612 - Physiology. Human and comparative physiology
dc.title Improving effects of long-term growth hormone treatment on monoaminergic neurotransmission and related behavioral tests in aged rats
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/acceptedVersion
dc.date.updated 2018-09-17T09:26:05Z
dc.date.embargoEndDate info:eu-repo/date/embargoEnd/2075-01-01
dc.embargo 2075-01-01
dc.rights.accessRights info:eu-repo/semantics/embargoedAccess
dc.identifier.doi https://doi.org/10.1089/rej.2010.1053


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