Ethanol desensitizes cannabinoid CB1 receptors modulating monoamines synthesis in the rat brain in vivo

Show simple item record Moranta Mesquida, David Esteban Valdés, Susana Cristina García-Sevilla, Jesús Andrés 2018-09-18T09:05:38Z
dc.description.abstract [eng] The endocannabinoid system and the cannabinoid CB1 receptors are involved in the development of ethanol tolerance and dependence. This study aimed to investigate the in vivo sensitivity of a CB1 receptor agonist (WIN 55,212-2) modulating the synthesis of 3,4-dihydroxyphenylalanine/dopamine/noradrenaline (DOPA/DA/NA) and that of 5-hydroxy-tryptophan/serotonin (5-HTP/5-HT) in rat brain after ethanol treatment and withdrawal. In control rats, WIN 55,212-2 (4 mg/kg, i.p., for 1 h), through a mechanism sensible to the CB1 antagonist SR 141716A, increased the synthesis of DOPA/NA in a slice of brainstem containing the locus ceruleus (250%) and in the hippocampus (64%), and it reduced DOPA/DA synthesis in the striatum (47%). WIN 55,212-2 also decreased the synthesis of 5-HTP/5-HT in the locus ceruleus (43%), hippocampus (35%) and striatum (35%). In the locus ceruleus of ethanol-treated rats, the stimulatory effect of WIN 55,212-2 on DOPA/NA synthesis was abolished (acute treatment) or markedly attenuated (53-55%, chronic treatment and withdrawal), whereas in the hippocampus this effect was reduced only in chronic ethanol-withdrawn rats (33%). In the striatum of ethanol-treated rats (acute, chronic and withdrawal), the inhibitory effect of WIN 55,212-2 on DOPA/DA synthesis was completely blunted or markedly reduced. Similarly, the inhibitory effect of WIN 55,212-2 on 5-HTP/5-HT synthesis was reduced or abolished in the three brain regions after chronic ethanol and during withdrawal. These results indicate that treatment with ethanol in rats induces a functional desensitization of CB1 receptors modulating the synthesis of brain monoamines.
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dc.relation.isformatof Versió postprint del document publicat a:
dc.relation.ispartof Neuroscience Letters, 2006, vol. 392, num. (1-2), p. 58-61
dc.rights (c) Elsevier Ireland Ltd., 2006
dc.subject.classification 612 - Fisiologia
dc.subject.classification 615 - Farmacologia. Terapèutica. Toxicologia. Radiologia
dc.subject.other 612 - Physiology. Human and comparative physiology
dc.subject.other 615 - Pharmacology. Therapeutics. Toxicology
dc.title Ethanol desensitizes cannabinoid CB1 receptors modulating monoamines synthesis in the rat brain in vivo
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/acceptedVersion 2018-09-18T09:05:38Z info:eu-repo/date/embargoEnd/2075-01
dc.embargo 2075-01
dc.subject.keywords etanol
dc.subject.keywords receptors per a serotonina
dc.subject.keywords síndrome de abstinència
dc.rights.accessRights info:eu-repo/semantics/embargoedAccess

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