Fully automatic flow-based device for monitoring of drug permeation across a cell monolayer

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dc.contributor.author Zelená Lucie
dc.contributor.author Marques, Sara S.
dc.contributor.author Segundo, Marcela A.
dc.contributor.author Miró Lladó, Manuel
dc.contributor.author Pávek, Petr
dc.contributor.author Sklenářová, Hana
dc.contributor.author Solich, Petr
dc.date.accessioned 2018-10-29T11:19:20Z
dc.identifier.uri http://hdl.handle.net/11201/148312
dc.description.abstract eng] A novel flow-programming setup based on the sequential injection principle is herein proposed for on-line monitoring of temporal events in cell permeation studies. The permeation unit consists of a Franz cell with its basolateral compartment mixed under mechanical agitation and thermostated at 37ºC. The apical compartment is replaced by commercially available Transwell® inserts with a pre-cultivated cell monolayer. The transport of drug substances across epithelial cells genetically modified with the P-glycoprotein membrane transporter (MDCKII - MDR1) is monitored on-line using Rhodamine 123 as a fluorescent marker. The permeation kinetics of the marker is obtained in a fully automated mode by sampling minute volumes of solution from the basolateral compartment in short intervals (10 min) up to 4 hours. The effect of a P-glycoprotein transporter inhibitor, verapamil as a model drug, on the efficiency of the marker transport across the cell monolayer is thoroughly investigated. The analytical features of the proposed flow method for cell permeation studies at real time are critically compared against conventional batch-wise procedures and microfluidic devices.
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dc.relation.isformatof Versió postprint del document publicat a:
dc.relation.ispartof Analytical and Bioanalytical Chemistry, 2016, vol. 408, p. 971-981
dc.rights (c) Springer-Verlag Berlin Heidelberg, 2016
dc.subject.classification 54 - Química
dc.subject.other 54 - Chemistry. Crystallography. Mineralogy
dc.title Fully automatic flow-based device for monitoring of drug permeation across a cell monolayer
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/acceptedVersion
dc.date.updated 2018-10-29T11:19:20Z
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dc.rights.accessRights info:eu-repo/semantics/embargoedAccess


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