Role of bacterial surface structures on the interaction of Klebsiella pneumoniae with phagocytes.

Show simple item record March, Catalina Cano, Victoria Moranta Mesquida, David Llobet Brossa, Enrique Pérez-Gutiérrez, Camino Tomás, Juan M. Suárez, Teresa Garmendia, Junkal Bengoechea Alonso, José Antonio 2015-09-25T11:51:23Z 2015-09-25T11:51:23Z 2013-02
dc.identifier.citation 1932-6203
dc.description.abstract Phagocytosis is a key process of the immune system. The human pathogen Klebsiella pneumoniae is a well known example of a pathogen highly resistant to phagocytosis. A wealth of evidence demonstrates that the capsule polysaccharide (CPS) plays a crucial role in resistance to phagocytosis. The amoeba Dictyostelium discoideum shares with mammalian macrophages the ability to phagocytose and kill bacteria. The fact that K. pneumoniae is ubiquitous in nature and, therefore, should avoid predation by amoebae, poses the question whether K. pneumoniae employs similar means to counteract amoebae and mammalian phagocytes. Here we developed an assay to evaluate K. pneumoniae-D. discoideum interaction. The richness of the growth medium affected the threshold at which the cps mutant was permissive for Dictyostelium and only at lower nutrient concentrations the cps mutant was susceptible to predation by amoebae. Given the critical role of bacterial surface elements on host-pathogen interactions, we explored the possible contribution of the lipopolysaccharide (LPS) and outer membrane proteins (OMPs) to combat phagoyctosis by D. discoideum. We uncover that, in addition to the CPS, the LPS O-polysaccharide and the first core sugar participate in Klebsiella resistance to predation by D. discoideum. K. pneumoniae LPS lipid A decorations are also necessary to avoid predation by amoebae although PagP-dependent palmitoylation plays a more important role than the lipid A modification with aminoarabinose. Mutants lacking OMPs OmpA or OmpK36 were also permissive for D. discoideium growth. Except the LPS O-polysaccharide mutants, all mutants were more susceptible to phagocytosis by mouse alveolar macrophages. Finally, we found a correlation between virulence, using the pneumonia mouse model, and resistance to phagocytosis. Altogether, this work reveals novel K. pneumoniae determinants involved in resistance to phagocytosis and supports the notion that Dictyostelium amoebae might be useful as host model to measure K. pneumoniae virulence and not only phagocytosis.
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.isformatof Reproducció del document publicat a: 10.1371/journal.pone.0056847
dc.relation.ispartof Plos One, 2013, vol. 8, num. 2, p. e56847
dc.rights cc-by (c) March, Catalina et al., 2013
dc.title Role of bacterial surface structures on the interaction of Klebsiella pneumoniae with phagocytes.
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion 2015-09-25T11:51:23Z
dc.rights.accessRights info:eu-repo/semantics/openAccess

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cc-by (c) March, Catalina et al., 2013 Except where otherwise noted, this item's license is described as cc-by (c) March, Catalina et al., 2013

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