Effects of hop-derived prenylflavonoids on mitochondrial function of SW620 colon cancer cells

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dc.contributor Sastre Serra, Jorge
dc.contributor.author Ahmiane, Youssef
dc.date 2018
dc.date.accessioned 2019-07-22T11:28:56Z
dc.date.issued 2018-02-22
dc.identifier.uri http://hdl.handle.net/11201/149678
dc.description.abstract [eng] Xanthohumol (XN), isoxanthohumol (IXN) and 8-prenylnaringenin (8-PN) are hop-derived prenylflavonoids and have been reported to exhibit anticancer properties in several types of cancer. They present a great interest against colon cancer due to high exposure of these compounds in this tissue. Metastatic SW-620 cell line was treated with doses ranging from 0.001 to 10 µM of XN, IXN or 8-PN in order to assess their effects on cell viability and mitochondrial function determined by Hoechst 33342, Amplex Red, MitoTracker® Green and Western blot. At low concentrations, these compounds had no effect on assays carried out. At high concentration, XN and IXN led to a decrease in cell viability, while the 8-PN had no effects. In addition, at 10 μM XN, it gave rise to an increase in ROS production accompanied by a decrease in OXPHOS complexes and sirtuin expression levels. These results suggest that XN or IXN in low dose and 8-PN do not alter the viability or mitochondrial function, whereas a high dose of XN induce mitochondrial dysfunction in SW620 colon cancer lines. ca
dc.format application/pdf
dc.publisher Universitat de les Illes Balears
dc.rights all rights reserved
dc.rights info:eu-repo/semantics/openAccess
dc.subject 577 - Bioquímica. Biologia molecular. Biofísica ca
dc.subject 616 - Patologia. Medicina clínica. Oncologia ca
dc.subject.other Colon cancer ca
dc.subject.other SW620 ca
dc.subject.other Xanthohumol ca
dc.subject.other Isoxanthohumol ca
dc.subject.other 8‐prenylnaringenin ca
dc.subject.other Mitochondria ca
dc.subject.other Mitochondrial function ca
dc.subject.other Oxidative phosphorylation system ca
dc.subject.other Sirtuins ca
dc.title Effects of hop-derived prenylflavonoids on mitochondrial function of SW620 colon cancer cells ca
dc.type info:eu-repo/semantics/masterThesis ca
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-12-20T09:42:45Z
dc.date.embargoEndDate info:eu-repo/date/embargoEnd/2050-01-01
dc.embargo 2050-01-01
dc.rights.accessRights info:eu-repo/semantics/embargoedAccess

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