Flow-through dynamic extraction system for exploring chyme bioaccessibility in food commodities

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dc.contributor.author Souza, Lais A
dc.contributor.author Rosende, María
dc.contributor.author Korn, Maria Graças A.
dc.contributor.author Miró, Manuel
dc.date.accessioned 2019-09-10T10:17:15Z
dc.identifier.uri http://hdl.handle.net/11201/149835
dc.description.abstract An automatic flow-through dynamic extraction method is proposed for the first time for in vitro exploration, with high temporal resolution, of the transit of the chyme from the gastric to the duodenal compartment using the Versantvoort's fed-state physiologically relevant extraction test. The flow manifold was coupled on-line to an inductively coupled plasma optical emission spectrometer (ICP OES) for real-time elucidation of the bioaccessible elemental fraction of micronutrients (viz., Cu, Fe and Mn) in food commodities across the gastrointestinal tract. The simulated intestinal and bile biofluid (added to the gastric phase) was successively pumped at 1.0 mL min−1 through a large-bore column (maintained at 37.0 ± 2.0 °C) initially loaded with a weighed amount of linseed (250 mg) using a PVDF filter membrane (5.0 μm pore size) for retaining of the solid sample and in-line filtration of the extracts. The lack of bias (trueness) of the on-line gastrointestinal extraction method coupled to ICP OES was confirmed using mass balance validation following microwave assisted digestion of the residual (non-bioaccessible) elemental fraction. Mass balance validation yielded absolute recoveries spanning from 79 to 121% for the overall analytes and samples. On-line dynamic extraction was critically appraised against batch counterparts for both gastric and gastrointestinal compartments. Due to the lack of consensus in the literature regarding the agitation method for batch oral bioaccessibility testing, several extraction approaches (viz., magnetic stirring, end-over-end rotation and orbital shaking) were evaluated. Improved gastric extractability of Fe along with bioaccessible data comparable to the dynamic counterpart based on the continuous displacement of the extraction equilibrium was obtained with batchwise magnetic stirring, which is deemed most appropriate for ascertaining worst-case/maximum bioaccessibility scenarios.
dc.format application/pdf
dc.relation.isformatof Versió postprint del document publicat a: https://doi.org/10.1016/j.aca.2018.04.067
dc.relation.ispartof Analytica Chimica Acta, 2018, vol. 1026, p. 51-61
dc.rights (c) Elsevier B.V., 2018
dc.subject.classification 543 - Química analítica
dc.subject.other 543 - Analytical chemistry
dc.title Flow-through dynamic extraction system for exploring chyme bioaccessibility in food commodities
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/acceptedVersion
dc.date.updated 2019-09-10T10:17:15Z
dc.date.embargoEndDate info:eu-repo/date/embargoEnd/2020-10-05
dc.embargo 2020-10-05
dc.rights.accessRights info:eu-repo/semantics/embargoedAccess
dc.identifier.doi https://doi.org/10.1016/j.aca.2018.04.067

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