Proteotyping bacteria: Characterization, differentiation and identification of pneumococcus and other species within the Mitis Group of the genus Streptococcus by tandem mass spectrometry proteomics

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dc.contributor.author Karlsson, Roger
dc.contributor.author Gonzales-Siles, Lucia
dc.contributor.author Gomila, Margarita
dc.contributor.author Busquets, Antonio
dc.contributor.author Salvà-Serra, Francisco
dc.contributor.author Jaén-Luchoro, Daniel
dc.contributor.author Jakobsson, Hedvig E.
dc.contributor.author Karlsson, Anders
dc.contributor.author Boulund, Fredrik
dc.contributor.author Kristiansson, Erik
dc.contributor.author Moore, Edward R. B.
dc.date.accessioned 2019-11-07T09:42:39Z
dc.date.available 2019-11-07T09:42:39Z
dc.identifier.uri http://hdl.handle.net/11201/150274
dc.description.abstract [eng] A range of methodologies may be used for analyzing bacteria, depending on the purpose and the level of resolution needed. The capability for recognition of species distinctions within the complex spectrum of bacterial diversity is necessary for progress in microbiological research. In clinical settings, accurate, rapid and cost-effective methods are essential for early and efficient treatment of infections. Characterization and identification of microorganisms, using, bottom-up proteomics, or 'proteotyping', relies on recognition of species-unique or associated peptides, by tandem mass spectrometry analyses, dependent upon an accurate and comprehensive foundation of genome sequence data, allowing for differentiation of species, at amino acid-level resolution. In this study, the high resolution and accuracy of MS/MS-based proteotyping was demonstrated, through analyses of the three phylogenetically and taxonomically most closely-related species of the Mitis Group of the genus Streptococcus: i.e., the pathogenic species, Streptococcus pneumoniae (pneumococcus), and the commensal species, Streptococcus pseudopneumoniae and Streptococcus mitis. To achieve high accuracy, a genome sequence database used for matching peptides was created and carefully curated. Here, MS-based, bottom-up proteotyping was observed and confirmed to attain the level of resolution necessary for differentiating and identifying the most-closely related bacterial species, as demonstrated by analyses of species of the Streptococcus Mitis Group, even when S. pneumoniae were mixed with S. pseudopneumoniae and S. mitis, by matching and identifying more than 200 unique peptides for each species.
dc.format application/pdf
dc.relation.isformatof Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0208804
dc.relation.ispartof Plos One, 2018, vol. 13, num. 12, p. 1-21
dc.rights cc-by (c) Karlsson, Roger et al., 2018
dc.rights.uri http://creativecommons.org/licenses/by/3.0/es
dc.subject.classification 575 - Genètica general. Citogenètica general. Immunogenètica. Evolució. Filogènia
dc.subject.other 575 - General genetics. General cytogenetics Immunogenetics. Evolution. Phylogeny
dc.title Proteotyping bacteria: Characterization, differentiation and identification of pneumococcus and other species within the Mitis Group of the genus Streptococcus by tandem mass spectrometry proteomics
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2019-11-07T09:42:39Z
dc.subject.keywords Proteotyping
dc.subject.keywords proteomics
dc.subject.keywords Genomics
dc.subject.keywords Streptococcus
dc.subject.keywords Streptococcus pneumoniae
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.identifier.doi https://doi.org/10.1371/journal.pone.0208804


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cc-by (c) Karlsson, Roger et al., 2018 Except where otherwise noted, this item's license is described as cc-by (c) Karlsson, Roger et al., 2018

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