dc.contributor.author |
García-Fuster, M. J. |
|
dc.contributor.author |
Ferrer-Alcón, M. |
|
dc.contributor.author |
Miralles, A. |
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dc.contributor.author |
García-Sevilla, J. A. |
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dc.date.accessioned |
2020-01-09T08:43:51Z |
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dc.date.available |
2020-01-09T08:43:51Z |
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dc.identifier.uri |
http://hdl.handle.net/11201/150532 |
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dc.description.abstract |
[eng] The Fas receptor is involved in the regulation of apoptosis but also can function as a non-apoptotic signal transducer. This study was mainly designed to quantitate Fas proteins in rat brain during heroin addiction and opiate withdrawal. In rat, mouse and human brains, and in SH-SY5Y cells, similar forms of Fas were immunodetected with different antibodies (i.e., 35 kDa native Fas and 48- and 51-kDa glycosylated Fas). Acute (2 h) treatments with the μ-opioid receptor agonists heroin (10 mg/kg) and morphine (30 mg/kg) increased the immunodensity of native Fas (124% and 36%) but not that of glycosylated Fas in the cerebral cortex. Chronic (5 days) heroin (5-30 mg/kg) and morphine (10-100 mg/kg) were also associated with increased native Fas (76% and 45%) and with different expressions of glycosylated Fas. In heroin-dependent rats, opiate withdrawal (48 h) resulted in a sustained increase in native Fas (107%) and in up-regulation of 51 kDa glycosylated Fas (51%). Acute treatments with selective δ-receptor (SNC-80, 10 mg/kg) or κ-receptor (U 50488-H, 10 mg/kg) agonists did not alter the content of native or glycosylated Fas. Chronic pentazocine (10-80 mg/kg, 5 days), a mixed opiate drug and σ1 receptor agonist, decreased native (48%) and glycosylated (38-82%) Fas proteins. Similarly, the selective σ1 agonist (+)-SKF 10047 also decreased native Fas (37%) and the effect was blocked by the σ1 antagonist BD 1063. Brain dynamin was up-regulated by acute and/or chronic heroin (30-39%), morphine (47-85%), pentazocine (51%) and heroin withdrawal (74%). The main results indicate that chronic heroin/morphine treatment and heroin withdrawal are associated with up-regulation of 35 kDa native Fas (and with different expressions of glycosylated Fas), and also with concomitant increases of dynamin in rat brain. |
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dc.format |
application/pdf |
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dc.relation.isformatof |
Versió postprint del document publicat a: https://doi.org/10.1007/s00210-003-0801-9 |
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dc.relation.ispartof |
Naunyn-Schmiedebergs Archives of Pharmacology, 2003, vol. 368, num. 5, p. 421-431 |
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dc.rights |
(c) Springer-Verlag, 2003 |
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dc.subject.classification |
615 - Farmacologia. Terapèutica. Toxicologia. Radiologia |
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dc.subject.other |
615 - Pharmacology. Therapeutics. Toxicology |
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dc.title |
Modulation of Fas receptor proteins and dynamin during opiate addiction and induction of opiate withdrawal in rat brain |
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dc.type |
info:eu-repo/semantics/article |
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dc.type |
info:eu-repo/semantics/acceptedVersion |
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dc.date.updated |
2020-01-09T08:43:51Z |
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dc.rights.accessRights |
info:eu-repo/semantics/openAccess |
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dc.identifier.doi |
https://doi.org/10.1007/s00210-003-0801-9 |
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