Cryopreservation of platelets treated with riboflavin and UV light and stored at −80°C for 1 year

Show simple item record Jimenez‐Marco, Teresa Ballester‐Servera, Carme Quetglas‐Oliver, Miguel Morell‐Garcia, Daniel Torres‐Reverte, Natalia Bautista‐Gili, Antonia M. Serra‐Ramon, Neus Girona‐Llobera, Enrique 2021-04-22T10:50:18Z
dc.description.abstract [eng] The combination of pathogen reduction technologies (PRTs) and cryopreservation can contribute to building a safe and durable platelet (PLT) inventory. Information about cryopreserved riboflavin and UV light‐treated PLTs is scarce. Study Design and Methods: Twenty‐four buffy coat (BC) PLT concentrates were grouped into 12 type‐matched pairs, pooled, and divided into 12 non‐PRT‐treated control units and 12 riboflavin and UV light PRT‐treated test units. Both were cryopreserved with 5% DMSO and stored at −80°C for 1 year. The cryopreservation method used was designed to avoid the formation of aggregates. PLT variables (PLT recovery, swirling, pH, MPV, and LDH) and hemostatic function measured by thromboelastography (TEG) were analyzed before cryopreservation (day 1) and post‐cryopreservation at day 14 and months 3, 6, and 12 of storage at −80°C. The analyses were carried out within 1‐h post‐thaw. Results: No aggregates were found in either PLT group at any time. Swirling was observed in both groups. MPV increased and mean pH values decreased over time (p <.001), but the mean pH value was never below 6.4 in either group after 12 months of storage at −80°C. PLT recovery was good and clotting time became significantly shorter over the storage period in both groups (p <.001). Conclusion: Our cryopreservation and thawing method prevented aggregate formation in cryopreserved riboflavin‐UV‐light‐treated PLTs, which exhibited good recovery, swirling, pH > 6.4, and procoagulant potential, as evidenced by a reduced clotting time after 12 months of storage at −80°C. The clinical relevance of these findings should be further investigated in clinical trials.
dc.format application/pdf
dc.relation.ispartof Transfusion, 2021, vol. 61, num. 4, p. 1235-1246
dc.rights , 2021
dc.subject.classification 61 - Medicina
dc.subject.other 61 - Medical sciences
dc.title Cryopreservation of platelets treated with riboflavin and UV light and stored at −80°C for 1 year
dc.type info:eu-repo/semantics/article 2021-04-22T10:50:18Z info:eu-repo/date/embargoEnd/2026-12-31
dc.embargo 2026-12-31
dc.rights.accessRights info:eu-repo/semantics/embargoedAccess

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