Effect of SIRT3 silencing on inflammation in breast cancer cell lines

Abstract

[eng] Sirtuin 3 (SIRT3) is the main mitochondrial deacetylase and through this deacetylation, it is able to regulate the activity of metabolic enzymes by modulating, among many other things, the production of reactive oxygen species (ROS). Recent studies suggest that SIRT3 could also participate in the regulation of inflammatory processes. The aim of this study was to analyze the relationship between SIRT3 silencing and inflammation markers in breast cancer cell lines. For this purpose, breast cancer cell lines MCF7 were transfected with a specific siRNA against SIRT3 to achieve the desired silencing. Then, gene expression of several proteins related with inflammation processes were determined using RT-qPCR. The genes studied included IL6, IL6R, IL8, IL8R, TNFa, TGFb, VEGFa, VEGFb, NF-kB and HPSE. SIRT3 silencing resulted in a significant increase in IL6 and a significant decrease in TGFb expression. In the rest of inflammation markers, no significant modification of expression was observed. In conclusion, with these results the hypothesis of a possible relationship between SIRT3 and inflammation in this type of cancer should be rejected. However, it is important that this silencing has led to a large increase in the expression of Interleukin 6, which is one of the key cytokines for the progression of breast cancer and could be targeted as a possible therapy