Legionella maioricensis sp. nov., a new species isolated from the hot water distribution systems of a hospital and a shopping center during routine sampling

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dc.contributor.author Crespi, Sebastian
dc.contributor.author Dra ar, Vladimír
dc.contributor.author Salvà-Serra, Francisco
dc.contributor.author Jaén-Luchoro, Daniel
dc.contributor.author Piñeiro-Iglesias, Beatriz
dc.contributor.author Christoffer Lindemann, Paul
dc.contributor.author Aliaga-Lozano, Francisco
dc.contributor.author Fernández-Juárez, Victor
dc.contributor.author Coll-García, Guillem
dc.contributor.author Moore, Edward R. B.
dc.contributor.author Bennasar-Figueras, Antoni
dc.date.accessioned 2023-03-15T12:30:37Z
dc.date.available 2023-03-15T12:30:37Z
dc.identifier.uri http://hdl.handle.net/11201/160303
dc.description.abstract [eng] Two Legionella-like strains isolated from hot water distribution systems in 2012 have been characterized phenotypically, biochemically and genomically in terms of DNA relatedness. Both strains, HCPI-6T and EUR-108, exhibited biochemical phenotypic profiles typical of Legionella species. Cells were Gram-negative motile rods which grew on BCYEα agar but not on blood agar and displayed phenotypic characteristics typical of the family Legionellaceae, including a requirement for l-cysteine and testing catalase positive. Both strains were negative for oxidase, urease, nitrate reduction and hippurate negative, and non-fermentative. The major ubiquinone was Q12 (59.4 % HCPI-6T) and the dominant fatty acids were C16 : 1 ω7c (28.4 % HCPI-6T, ≈16 % EUR-108), C16 : 0 iso (≈22.5 % and ≈13 %) and C15 : 0 anteiso (19.5 % and ≈23.5 %, respectively). The percent G+C content of genomic DNA was determined to be 39.3 mol %. The 16S rRNA gene, mip sequence and comparative genome sequence-based analyses (average nucleotide identity, ANI; digital DNA-DNA hybridization, dDDH; and phylogenomic treeing) demonstrated that the strains represent a new species of the genus Legionella. The analysis based on the 16S rRNA gene sequences showed that the sequence similarities for both strains ranged from 98.8-90.1 % to other members of the genus. The core genome-based phylogenomic tree (protein-concatemer tree based on concatenation of 418 proteins present in single copy) revealed that these two strains clearly form a separate cluster within the genus Legionella. ANI and dDDH values confirmed the distinctiveness of the strains. Based on the genomic, genotypic and phenotypic findings from a polyphasic study, the isolates are considered to represent a single novel species, for which the name Legionella maioricensis sp. nov. is proposed. The type strain is HCPI-6T (=CCUG 75071T=CECT 30569T).
dc.format application/pdf
dc.relation.isformatof Versió postprint del document publicat a: https://doi.org/10.1099/ijsem.0.005686
dc.relation.ispartof International Journal of Systematic and Evolutionary Microbiology, 2023, vol. 73, num. 1, p. 005686
dc.rights (c) International Union of Microbiological Societies (IUMS), 2023
dc.subject.classification 57 - Biologia
dc.subject.other 57 - Biological sciences in general
dc.title Legionella maioricensis sp. nov., a new species isolated from the hot water distribution systems of a hospital and a shopping center during routine sampling
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/acceptedVersion
dc.date.updated 2023-03-15T12:30:38Z
dc.subject.keywords Legionella
dc.subject.keywords Legionellosis
dc.subject.keywords taxonomy
dc.subject.keywords Novel species
dc.subject.keywords Phylogenomics
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.identifier.doi https://doi.org/10.1099/ijsem.0.005686

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