A Sequential Micro-Immunotherapy Medicine Increases Collagen Deposition in Human Gingival Fibroblasts and in an Engineered 3D Gingival Model under Inflammatory Conditions

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dc.contributor.author Ferrà-Cañellas, Maria del Mar
dc.contributor.author Munar-Bestard, Marta
dc.contributor.author Floris, Ilaria
dc.contributor.author Ramis, Joana Maria
dc.contributor.author Monjo, Marta
dc.contributor.author Garcia-Sureda, Laura
dc.date.accessioned 2023-11-10T08:22:10Z
dc.date.available 2023-11-10T08:22:10Z
dc.identifier.uri http://hdl.handle.net/11201/162764
dc.description.abstract [eng] Periodontal therapies use immune mediators, but their side effects can increase with dosage. Micro-immunotherapy (MI) is a promising alternative that employs immune regulators at low and ultralow doses to minimize adverse effects. In this study, the effects of 5 capsules and the entire 10-capsule sequence of the sequential MI medicine (MIM-seq) were tested in two in vitro models of periodontitis. Firstly, human gingival fibroblasts (hGFs) exposed to interleukin (IL)-1β to induce inflammation were treated with five different capsules of MIM-seq for 3 days or with MIM-seq for 24 days. Subsequently, MIM-seq was analyzed in a 3D model of human tissue equivalent of gingiva (GTE) under the same inflammatory stimulus. Simultaneously, a non-IL-1β-treated control and a vehicle were included. The effects of the treatments on cytotoxicity, collagen deposition, and the secreted levels of IL-1α, IL-6, prostaglandin E2 (PGE2), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were evaluated. None of the tested items were cytotoxic. The complete sequence of MIM-seq decreased PGE2 release and restored collagen deposition levels induced by IL-1β treatment in hGFs exposed to IL-1β. MIM-seq treatment restored collagen production levels in both models. These promising preclinical findings suggest that MIM-seq should be further investigated for periodontitis treatment.
dc.format application/pdf
dc.relation.isformatof Reproducció del document publicat a: https://doi.org/10.3390/ijms241310484
dc.relation.ispartof International Journal Of Molecular Sciences, 2023, vol. 24, num. 13, p. 10484-1-10484-16
dc.rights cc-by (c) Ferrà-Cañellas, Maria del Mar et al., 2023
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject.classification 57 - Biologia
dc.subject.classification Ciències de la salut
dc.subject.other 57 - Biological sciences in general
dc.subject.other Medical sciences
dc.title A Sequential Micro-Immunotherapy Medicine Increases Collagen Deposition in Human Gingival Fibroblasts and in an Engineered 3D Gingival Model under Inflammatory Conditions
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2023-11-10T08:22:11Z
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.identifier.doi https://doi.org/10.3390/ijms241310484


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cc-by (c) Ferrà-Cañellas, Maria del Mar et al., 2023 Except where otherwise noted, this item's license is described as cc-by (c) Ferrà-Cañellas, Maria del Mar et al., 2023

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