dc.contributor.author | Ferrà-Cañellas, Maria del Mar | |
dc.contributor.author | Munar-Bestard, Marta | |
dc.contributor.author | Floris, Ilaria | |
dc.contributor.author | Ramis, Joana Maria | |
dc.contributor.author | Monjo, Marta | |
dc.contributor.author | Garcia-Sureda, Laura | |
dc.date.accessioned | 2023-11-10T08:22:10Z | |
dc.date.available | 2023-11-10T08:22:10Z | |
dc.identifier.uri | http://hdl.handle.net/11201/162764 | |
dc.description.abstract | [eng] Periodontal therapies use immune mediators, but their side effects can increase with dosage. Micro-immunotherapy (MI) is a promising alternative that employs immune regulators at low and ultralow doses to minimize adverse effects. In this study, the effects of 5 capsules and the entire 10-capsule sequence of the sequential MI medicine (MIM-seq) were tested in two in vitro models of periodontitis. Firstly, human gingival fibroblasts (hGFs) exposed to interleukin (IL)-1β to induce inflammation were treated with five different capsules of MIM-seq for 3 days or with MIM-seq for 24 days. Subsequently, MIM-seq was analyzed in a 3D model of human tissue equivalent of gingiva (GTE) under the same inflammatory stimulus. Simultaneously, a non-IL-1β-treated control and a vehicle were included. The effects of the treatments on cytotoxicity, collagen deposition, and the secreted levels of IL-1α, IL-6, prostaglandin E2 (PGE2), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were evaluated. None of the tested items were cytotoxic. The complete sequence of MIM-seq decreased PGE2 release and restored collagen deposition levels induced by IL-1β treatment in hGFs exposed to IL-1β. MIM-seq treatment restored collagen production levels in both models. These promising preclinical findings suggest that MIM-seq should be further investigated for periodontitis treatment. | |
dc.format | application/pdf | |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/ijms241310484 | |
dc.relation.ispartof | International Journal Of Molecular Sciences, 2023, vol. 24, num. 13, p. 10484-1-10484-16 | |
dc.rights | cc-by (c) Ferrà-Cañellas, Maria del Mar et al., 2023 | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject.classification | 57 - Biologia | |
dc.subject.classification | Ciències de la salut | |
dc.subject.other | 57 - Biological sciences in general | |
dc.subject.other | Medical sciences | |
dc.title | A Sequential Micro-Immunotherapy Medicine Increases Collagen Deposition in Human Gingival Fibroblasts and in an Engineered 3D Gingival Model under Inflammatory Conditions | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.date.updated | 2023-11-10T08:22:11Z | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
dc.identifier.doi | https://doi.org/10.3390/ijms241310484 |
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