Comparative proteogenomics of twelve Roseobacter exoproteomes reveals different adaptive strategies amongst these marine bacteria

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dc.contributor.author Christie-Oleza, J.A.
dc.contributor.author Piña-Villalonga, J.M.
dc.contributor.author Bosch, R.
dc.contributor.author Nogales, B.
dc.contributor.author Armengaud, J.
dc.date.accessioned 2025-01-17T12:23:14Z
dc.date.available 2025-01-17T12:23:14Z
dc.identifier.citation Christie-Oleza, J.A., Piña-Villalonga, J.M., Bosch, R., Nogales, B., Armengaud, J. (2012). Comparative proteogenomics of twelve Roseobacter exoproteomes reveals different adaptive strategies amongst these marine bacteria. Molecular & Cellular Proteomics, 11, 1-12
dc.identifier.uri http://hdl.handle.net/11201/167800
dc.description.abstract [eng] Roseobacters are generalist bacteria abundantly found in the oceans. Because little is known on how marine microorganisms interact in association or competition, we focused our attention on the microbial exoproteome, a key component in their interaction with extracellular milieu. Here we present a comparative analysis of the theoretically encoded exoproteome of twelve members of the Roseobacter group validated by extensive comparative proteogenomics. In silico analysis revealed that 30% of the encoded proteome of these microorganisms could be exported. The ratio of the different protein categories varied in accordance to the ecological distinctness of each strain, a trait reinforced by quantitative proteomics data. Despite the interspecies variations found, the most abundantly detected proteins by shotgun proteomics were from transporter, adhesion, motility, and toxin-like protein categories, defining four different plausible adaptive strategies within the Roseobacter group. In some strains the toxin-secretion strategy was over-represented with repeats-in-toxin-like proteins. Our results show that exoproteomes strongly depend on bacterial trophic strategy and can slightly change because of culture conditions. Simulated natural conditions and the effect of the indigenous microbial community on the exoproteome of Ruegeria pomeroyi DSS-3 were also assayed. Interestingly, we observed a significant depletion of the toxin-like proteins usually secreted by R. pomeroyi DSS-3 when grown in presence of a natural community sampled from a Mediterranean Sea port. The significance of this specific fraction of the exoproteome is discussed.
dc.format application/pdf
dc.format.extent 1-12
dc.publisher American Society for Biochemistry and Molecular Biology
dc.relation.ispartof Molecular & Cellular Proteomics, 2012, vol. 11, p. 1-12
dc.rights Attribution 4.0 International
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject.classification 57 - Biologia
dc.subject.classification 579 - Microbiologia
dc.subject.other 57 - Biological sciences in general
dc.subject.other 579 - Microbiology
dc.title Comparative proteogenomics of twelve Roseobacter exoproteomes reveals different adaptive strategies amongst these marine bacteria
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2025-01-17T12:23:14Z
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.identifier.doi https://doi.org/10.1074/mcp.M111.013110


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